Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP-UEA-Norwich
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:15
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K774004_sequence (Version 1) |
Description
Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: enhanced Cyan Fluorescence Protein (eCFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.Notes
The enhanced Cyan Fluorescence Protein (eCFP) was chosen with the eventual aim of attaching it to part BBa_K774002 or BBa_K774003. It was therefore important that the fluorescence peak of eCFP didn't overlap with RFP too much, as eCFP and RFP will be reporting on either part BBa_K774002 or BBa_K774003 at the same time.Source
BBaK216005 + BHN + CArG promoter sequence E9-ns2 + BBa_E0420| Access | Instance | Definition |
| public public | BBa_K774000 BBa_E0420 | B-M BBa_E0420 |