Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP-UEA-Norwich
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:15
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K774006_sequence (Version 1) |
Description
Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , in the orientation mammalian to bacterial, both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: enhanced Cyan Fluorescence Protein (eCFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.Notes
This biobrick contains the RBS and terminators, meaning we can simply add the promoter of choice to complete the system.Source
E9-ns2 CArG promoter sequence + BHN + BBaK216005| Access | Instance | Definition |
| public public | BBa_K774001 BBa_E0420 | M-B BBa_E0420 |