BBa_K775004

BBa_K775004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K775004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sietske Grijseels
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:16

GPCR reporter FUS1-EGFP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K775004_sequence (Version 1)

Description

This reporter can be used in combination with the expression of a G-protein coupled receptor in yeast. The Fus1 promoter is a promoter in the mating pathway (a G-protein coupled receptor pathway. If the receptor of this pathway is activated by the pheromone of the opposite mating type the mating pathway is activated and the Fus1 promoter is switched on.

Notes

-

Source

The sequence of the Fus1 promoter comes from S. cerevisiae

Sequence Annotation Location Component / Role(s)
FUS1 promoter
BamHi restriction site
Kozak sequence
EGFP
NdeI restriction site
ADH1 terminator
1,200
201,206
207,212
213,936
937,942
942,1166
feature/promoter promoter
sequence_feature feature/misc
feature/rbs ribosome_entry_site
feature/protein CDS
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K775004/1