Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Goettingen
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:16
fliC under the control of constitutive promoter J23100
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K777110_sequence (Version 1) |
Description
The fliC gene codes for flagellin. This protein is the subunit that forms the flagellar filament of E. coli.Here we used 3 different constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive fliC expression affect the motility or flagellum size of E. coli.
Notes
- The fliC gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
- fliC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: 5'-gtttcttcgaattcgcggccgcttctagatggcacaagtcattaatacc-3'
- Rev: 5'-gtttcttcgaattcgcggccgcttctagttaaccctgGagcagagacagaacc-3'
(the capitalized "G" induces the mutation for removal of the third PstI site)
Source
- The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
| Access | Instance | Definition |
| public public | BBa_J23100 BBa_K777109 | BBa_J23100 BBa_K777109 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K777109 | 1,35 42,1538 | BBa_J23100 BBa_K777109 |