BBa_K777121

BBa_K777121 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K777121
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Flash Coli
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:17

yhjH



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K777121_sequence (Version 1)

Description

The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in yhjH-mutants.

Notes

  • yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
    • Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'


Source

The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

Sequence Annotation Location Component / Role(s)
YhjH coding sequence
start
stop
1,768
1,1
768,768
feature/cds CDS
start_codon feature/start
stop_codon feature/stop
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
rdf-schema#seeAlso
P37646
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K777121/1