Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K777121_sequence (Version 1)
|
Description
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in yhjH-mutants.
Notes
- yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
- Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
Source
The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).