BBa_K777133

BBa_K777133 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K777133
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Goettingen
Date created: 2012-09-27 11:00:00
Date modified: 2015-05-08 01:13:17

Similar to pSB1C3, with RFP reporter



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_K777133_sequence (Version 1)

Description

This part is similar to J61002 but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.

Notes

  • No specific design considerations.


Source

  • This part is a compination of E1010, B0034 and pSB1C3 from the registry.


Sequence Annotation Location Component / Role(s)
RBS
BB0034
mRFP1
E1010
rep (pMB1)
Chloramphenicol resistance
ORI
VR primer binding site
VF2 primer binding site
SpeI
PstI
EcoRI
XbaI
8,19
8,19
26,703
26,703
1129,1735
2028,2687
1144,1144
1023,1042
2799,2818
1,6
884,889
2917,2922
2932,2937
ribosome_entry_site feature/rbs
engineered_region feature/BioBrick
feature/cds CDS
feature/BioBrick engineered_region
feature/misc sequence_feature
CDS feature/cds
sequence_feature feature/misc
primer_binding_site feature/primer_binding
primer_binding_site feature/primer_binding
feature/dna sequence_feature
sequence_feature feature/dna
feature/dna sequence_feature
feature/dna sequence_feature
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K777133/1