Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristjan Eerik Kaseniit
Date created: 2012-09-28 11:00:00
Date modified: 2015-05-08 01:13:18
Long reporter top strand with bulge MammoBlock
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K779500_sequence (Version 1) |
Description
Reporter top strand with a 4nt bulge.Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmC mAmCmU mUmCmU mCmCmC mAmCmC mA/3AlexF488N/
Notes
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.4-nucleotide bulge added 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
Source
De novo design.| Sequence Annotation | Location | Component / Role(s) |
| Bulge Domain Sb Domain Sa part 1 Domain Sa part 2 | 11,14 1,24 1,10 15,24 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |