Types | DnaRegion
|
Roles | Other
sequence_feature
|
Sequences | BBa_K779501_sequence (Version 1)
|
Description
Reporter top strand.
Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA /3AlexF488N/
Notes
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
Source
De novo design.