BBa_K780000

BBa_K780000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K780000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nikodem Latocha

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:18

Terminator for Bacillus subtilis

Details

• Types: DnaRegion
• Roles: Terminator; terminator
• Sequences: BBa_K780000_sequence (Version 1)

Description

Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils.
Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase.
Gyrase gene is an housekeeping gene and because of that it must have efficient terminator.

Notes

-

Source

Terminator of gyrase (EC 5.99.1.3) gene.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K780000/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //direction/forward
• //terminator/single
• iGEM Parts Registry