Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - RFP-FliC Insertion Chimera
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K781001_sequence (Version 1) |
Description
This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as an insertion.This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.
Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.Source
This was created using overlap extension PCR to insert into K781000 at the annotated site. The insert sequence was amplified using PCR from J04450.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_J04500 CAP -35 -10 LacI start BBa_B0034 conserved Flagellin 1st Overlapping region Linker BBa_E1010 Linker 2 Flagellin 2nd Overlapping region | 1,200 1,220 89,126 137,142 161,166 166,200 173,173 209,220 213,216 230,975 946,976 977,996 997,1672 1673,1692 1693,2443 1693,1721 | engineered_region feature/BioBrick engineered_region feature/BioBrick feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter feature/binding non_covalent_binding_site start_codon feature/start feature/BioBrick engineered_region feature/conserved conserved_region feature/protein CDS primer_binding_site feature/primer_binding CDS feature/protein feature/BioBrick engineered_region feature/protein CDS feature/protein CDS feature/primer_binding primer_binding_site |