Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - Rv2579 Dehalogenase-FliC Insertion Chimera
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K781004_sequence (Version 1) |
Description
LinB is a dehalogenase enzyme. This construct as incorporated that enzyme as an insertion into the variable domain.Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.Source
This part was PCR amplified from a plasmid provided by another research lab.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 Flagellin Linker 1 Rv2579 Linker 2 Flagellin | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 230,976 977,997 998,1888 1889,1908 1909,2659 | feature/BioBrick engineered_region feature/cds CDS non_covalent_binding_site feature/binding feature/promoter promoter promoter feature/promoter non_covalent_binding_site feature/binding feature/start start_codon feature/conserved conserved_region feature/BioBrick engineered_region CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein feature/protein CDS |