Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - RFP-FliC Deletion Chimera
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K781006_sequence (Version 1) |
Description
This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as a deletion.This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.
Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera. Note that there are two different formats for making flagellin insertion variants and deletion variantsSource
This part was PCR amplified from J04450 and then overlap extension was used to incorporate it into the plasmid.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 BBa_E1010 Flagellin Flagellin Linker 1 Linker 2 | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 808,1482 229,792 1496,2154 793,807 1483,1497 | engineered_region feature/BioBrick feature/cds CDS non_covalent_binding_site feature/binding feature/promoter promoter feature/promoter promoter feature/binding non_covalent_binding_site feature/start start_codon conserved_region feature/conserved engineered_region feature/BioBrick feature/BioBrick engineered_region feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS |