Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - XylE-FliC Deletion Chimera
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K781007_sequence (Version 1) |
Description
This is an XylE deletion variant of the flagellin monomer. The enzyme XylE that degrades catechol was incorporated into the E. coli flagellin by replacing the variable domain.Notes
When amplifying this part, the overlapping regions were carefully chosen so that the insert sequence alone can be inforporated into a plasmid, for future use in overlap extension. Digesting with the two inner enzymes for the BB-2 enzymes S and N, leaves the appropriate sequence for overlap extension.Source
The original XylE came from a biobrick part. It was amplified using PCR and then overlap extension was used to incorporate it into the flagella.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 Flagellin K147002 Flagellin Linker 1 Linker 2 | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 229,777 808,1728 1759,2403 778,807 1729,1758 | engineered_region feature/BioBrick CDS feature/cds non_covalent_binding_site feature/binding promoter feature/promoter feature/promoter promoter non_covalent_binding_site feature/binding feature/start start_codon feature/conserved conserved_region engineered_region feature/BioBrick feature/protein CDS engineered_region feature/BioBrick feature/protein CDS CDS feature/protein CDS feature/protein |