BBa_K783055

BBa_K783055 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K783055

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Traci Haddock

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:21

Level 0 MoClo Destination Vector with AB

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_K783055_sequence (Version 1)

Description

This is a Level 0 MoClo destination vector with flanking sites A on the 5' side and site B on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites:
A GGAG E GCTT
B TACT F CGCT
C AATG G TGCC
D AGGT H ACTA

Notes

We had to confirm the lacZ orientation was 5'->3' after cloning.

Source

We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K783055/1

Member of these Collections

• iGEM Parts Registry