BBa_K784005

BBa_K784005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K784005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-12 11:00:00
Date modified: 2015-05-08 01:13:21

Theophylline riboswitch 12.1



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K784005_sequence (Version 1)

Description

A riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.

Notes

The part must be directly fused to the starting codon of the downstream coding region.

Source

The part was obtained from the Gallivan lab. The description of the part was found in the following paper:
Lynch Sean A., Gallivan Justin P. 2009. A flow cytometry-based screen for synthetic riboswitches. Nucleic Acids Research 37(1): 184-192.

Sequence Annotation Location Component / Role(s)
Additional 5' UTR
Theophylline aptamer
RBS
1,17
18,55
56,74
feature/misc sequence_feature
sequence_feature feature/misc
ribosome_entry_site feature/rbs
igem#experience
Issues
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K784005/1