BBa_K784007

BBa_K784007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K784007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-14 11:00:00
Date modified: 2015-05-08 01:13:21

Spacer + RBS from theophylline riboswitch



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_K784007_sequence (Version 1)

Description

This part has been created by deleting the aptamer and the additional 5' UTR regions of the [[Part:BBa_K784005|theophylline riboswitch]] using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the [[Part:BBa_K784005|theophylline riboswitch]] remains.

Notes

The spacer region was designed to have minimal secondary structures. In particular, it was designed to have minimal base pairs in the with the RBS, in order to keep it free for interaction with the ribosome.
The PacI restriction site in the spacer region was used to self ligate the PCR product, which was a complete plasmid without the aptamer and the additional 5' UTR regions of the [[Part:BBa_K784005|theophylline riboswitch]].

Source

There is no source for this part. The RBS is the one used in the [[Part:BBa_K784005|theophylline riboswitch]].

Sequence Annotation Location Component / Role(s)
Spacer region
PacI restriction site
RBS
1,27
12,19
28,46
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K784007/1