Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-14 11:00:00
Date modified: 2015-05-08 01:13:21
MCS+tac promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K784008_sequence (Version 1) |
Description
The tac promoter is a functional hybrid promoter, derived from the trp and lac promoters. The sequence here is preceded by an MCS as a by product of the restriction cloning steps from a pUC19 plasmid which contained the original sequence. The promoter was followed by the [[Part:BBa_K784005|theophylline riboswitch]] and the complete construct was used for the Assembly pcr process with different proteins.Notes
There are no design considerations.Source
A pUC19 plasmid sent to us from the Gallivan lab. The promoter was followed by the [[Part:BBa_K784005|theophylline riboswitch]].| Sequence Annotation | Location | Component / Role(s) |
| MCS tac promoter | 1,21 22,61 | sequence_feature feature/misc sequence_feature feature/misc |