Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-14 11:00:00
Date modified: 2015-05-08 01:13:21
MCS+tac promoter+theophylline riboswitch+mCherry
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K784010_sequence (Version 1) |
Description
This is a construct containing an [[Part:BBa_K784008|MCS followed by a tac promoter]] followed by the [[Part:BBa_K784005|theophylline riboswitch]] fused to mCherry. The construct was created using Assembly pcr. This construct was used to characterize the relationship between the theophylline concentration and the level of translation of mCherry fused to the theophylline riboswitch.Notes
The coding region of the fused protein may have effect on the riboswitch function.Source
Assembly pcr product of [[Part:BBa_K784008|MCS+tac promoter]] and [[Part:BBa_K784005|theophylline riboswitch]].| Access | Instance | Definition |
| public public | BBa_K784009 BBa_J06504 | BBa_K784009 mCherry |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784009 BBa_J06504 | 1,765 766,1479 | BBa_K784009 mCherry |