Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-15 11:00:00
Date modified: 2015-05-08 01:13:21
Spacer2 + RBS from theophylline riboswitch
| Types | DnaRegion |
| Roles | RBS ribosome_entry_site |
| Sequences | BBa_K784012_sequence (Version 1) |
Description
This part has been created by deleting the aptamer and the additional 5' UTR regions of the [[Part:BBa_K784005|theophylline riboswitch]] using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the [[Part:BBa_K784005|theophylline riboswitch]] remains.This part is the result of an insertion mutation to [[Part:BBa_K784007|BBa_K784007]].
Notes
There is no source for this part. The RBS is the one used in the [[Part:BBa_K784005|theophylline riboswitch]].Source
The spacer region was designed to have minimal secondary structures. In particular, it was designed to have minimal base pairs in the with the RBS, in order to keep it free for interaction with the ribosome.The PacI restriction site in the spacer region was used to self ligate the PCR product, which was a complete plasmid without the aptamer and the additional 5' UTR regions of the [[Part:BBa_K784005|theophylline riboswitch]].