Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lior Levy
Date created: 2012-09-16 11:00:00
Date modified: 2015-05-08 01:13:22
pSP6+RBS+Alkaline Phosphatase (pHO)+Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K784030_sequence (Version 1) |
Description
This part consists of T7 RNA polymerase induced promoter followed by an RBS and the Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been made by Arkin Lab group in 2006.
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and therefore can be excised out by these enzymes.
Notes
we used XhoI and XmaI restriction sites for the ligation because these sites are not anywhere in the sequence.Source
The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.The RBS is from the parts registry,BBa_B0064 made by Endi Lab (2008)
The pHOA gene is from the parts registry,BBa_J61032 made by Arkin Lab (2006)
| Access | Instance | Definition |
| public public public | BBa_K784026 BBa_K784027 BBa_K784002 | BBa_K784026 BBa_K784027 BBa_K784002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784026 BBa_K784027 BBa_K784002 | 1,17 18,1516 1517,1563 | BBa_K784026 BBa_K784027 BBa_K784002 |