Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noa Katz
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:22
pK1F_xyIE reporter gene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K784033_sequence (Version 1) |
Description
This part's purpose is to testify to the presence of the K1F RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh (http://partsregistry.org/Part:BBa_J33204). It consists of an RNA K1F promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes.
Notes
We had to make sure to use restriction enzymes that don't have a restriction site in the gene itself.Source
The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.| Access | Instance | Definition |
| public public public | BBa_K784025 BBa_K784031 BBa_K784002 | BBa_K784025 BBa_K784031 BBa_K784002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784025 BBa_K784031 BBa_K784002 | 1,23 32,1007 1016,1062 | BBa_K784025 BBa_K784031 BBa_K784002 |