Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noa Katz
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:22
pSp6_xyIE reporter gene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K784034_sequence (Version 1) |
Description
This part's purpose is to testify to the presence of the Sp6 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh (http://partsregistry.org/Part:BBa_J33204). It consists of an RNA Sp6 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes.
Notes
We had to make sure there aren't any restriction sites in the gene itself before using the XhoI and XmaI restriction enzymes.Source
The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh.The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.
| Access | Instance | Definition |
| public public public | BBa_K784026 BBa_K784031 BBa_K784002 | BBa_K784026 BBa_K784031 BBa_K784002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784026 BBa_K784031 BBa_K784002 | 1,17 26,1001 1010,1056 | BBa_K784026 BBa_K784031 BBa_K784002 |