BBa_K784043

BBa_K784043 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K784043

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Inbal Vaknin

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:22

Mutant T7 RNAP - T7*

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_K784043_sequence (Version 1)

Description

This part includes an engineered T7 RNAP with a RBS, the RNAP recognizes specifically a suitable promoter (pT7). The mutant T7 RNAP was donated us by Chris Voight.
The T7 RNAP part contains a weak ribosome binding site and 'GTG' start codon .
The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn???t reduce the RNAP activity.

Notes

The cloning of this part included adding a restriction site at the beginning of the mutant T7 RNAP: XbaI at the 5' end, which was added by PCR (this was done to suit the RNAP for the standards). There wasn???t any necessity to add the suffix restriction sites at the 3' because it was already at the end of the RNAP.

Source

Temme K., et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	23,56	ribosome_entry_site, feature/rbs
T7*	57,2831	feature/cds, CDS
umu tag	58,176	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K784043/1

Member of these Collections

• iGEM Parts Registry