BBa_K784046

BBa_K784046 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K784046

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Asaf Blumental

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:22

theophylline riboswitch +T7*(N4) RNAP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K784046_sequence (Version 1)

Description

This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered N4 RNA polymerase (K784044). The riboswitch contains a RBS and it will be exposed and starts translating the N4 RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the N4 RNAP are adjacent to one another without any restriction site between them.

Notes

The riboswitch had to fused to the RNAP using fusion PCR.

Source

Fusion PCR of BBa K784005 and K784044

Components

Access	Instance	Definition
public	BBa_K784005	BBa_K784005
public	BBa_K784044	BBa_K784044

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K784005	1,74	BBa_K784005
BBa_K784044	75,2852	BBa_K784044

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K784046/1

Member of these Collections

• iGEM Parts Registry