Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zining Hou
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:22
Lov-tetR (light sensor with Ptet repression) light->PtetO repressed
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K785000_sequence (Version 1) |
Description
This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light.This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA.
We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.
Notes
This part is designed for our biowave project.Source
This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.| Sequence Annotation | Location | Component / Role(s) |
| Lov light sensor domain linker part tetR DNA binding domain | 1,294 213,345 294,1044 | CDS feature/cds sequence_feature feature/misc non_covalent_binding_site feature/binding |