Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung Wai Tak
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:22
Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K786002_sequence (Version 1) |
Description
Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm.We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Notes
Pfam (version 26.0) was used to predict the domain of fusion protein.Linker was refereed and modified from previous research study of SRII fusion with HtrII.
Source
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.| Sequence Annotation | Location | Component / Role(s) |
| Constitutive promoter BBa_J23100 RBS 1.0 HindIII for N-terminal fusin SRII-HtrII-Tar BamHI for C-terminal fusion B0015 | 1,41 42,61 62,67 68,2083 785,790 2117,2245 | feature/promoter promoter ribosome_entry_site feature/rbs feature/misc sequence_feature feature/protein CDS feature/misc sequence_feature feature/BioBrick engineered_region |