Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ChunHungKong, KenHo, LeonardoKan, Kaiyin Feng
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:22
T7-repeat-spacer-repeat-T7terminator
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K786030_sequence (Version 1) |
Description
It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system.Notes
The repeat-spacer-repeat region design should be consistent with the working principles of E.coli's CRISPR/cas system.Source
The 'repeat' sequence in CRISPR/cas system of E.coli strain K12.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I719005 BBa_K606028 T7 promoter repeat-spacer region | 1,23 129,264 1,23 31,120 | engineered_region feature/BioBrick feature/stop stop_codon feature/promoter promoter sequence_feature feature/misc |