PolyWb

BBa_K792008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K792008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Manuel Gim??nez

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:23

Tryptophan rich peptide (PolyWb)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K792008_sequence (Version 1)

Description

Stable and soluble Histidine rich peptide (designed in silico)
This part has a HindIII restriction site as extra.

Notes

PolyWb was desing taking into acount the following consideration:

1. avoided repeating the same residue in tandem to minimize local tRNA depleation
2. avoided Trp in tandem because of therir low solubility
3. we included Gly to avoid the formation of stable structures
4. included acidic and basic amino acids to increase solubility

Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.

Source

Designed by BsAs Team 2012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
HindIII restriction site	1,6	feature/misc, sequence_feature
stop	115,120	stop_codon, feature/stop
cds	7,114	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K792008/1

Member of these Collections

• //chassis/eukaryote/yeast
• //proteindomain/tail
• iGEM Parts Registry