Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tomohiro Nobeyama
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:23
Plasmid Backbone for golden gate assembly
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K797013_sequence (Version 1) |
Description
This backbone is suitable for golden gate assembly.Golden gate assembly is a suitable assembly to combine many parts in order you want.
This plasmid backbone have restriction enzyme cutting site of Dpn1 let you build construction by Golden gate assembly in one ligation.
Notes
The terminal of this part was modified to use it for Golden gate assemblySource
This part was made by PCR from iGEM part BBa_PSB1K3| Sequence Annotation | Location | Component / Role(s) |
| BsaI Recognition site BsaI Restriction site BioBrick suffix VR VF2 BioBrick prefix BsaI Restriction site BsaI Recognition site | 4,9 11,14 15,35 157,176 2101,2120 2199,2219 2219,2222 2224,2229 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/dna primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/dna sequence_feature feature/misc sequence_feature sequence_feature feature/misc |