Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ingmar Polte
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:24
pTUM100 yeast shuttle vector based on pYES2
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K801000_sequence (Version 1) |
Description
A yeast shuttle vector containing the following structural elements:- 2?? ori for replication in S. cerevisiae
- URA3-gene for selection of transformed yeast via Uracil prototrophy
- pUC ori for replication in E. coli
- AmpR-gene for selection of transformed E. coli via Ampicillin resistance
- f1 ori
- MCS containing the RFC25 pre- and suffix for easy insertion of biobricks
- following the MCS: CYC1 yeast transcriptional terminator
Note: To use this backbone, a S. cerevisiae strain with Uracil-auxotrophy (such as strain INVSc1) is required for positive selection of transformants. Successfully transformed yeast cells will become prototrophic for uracil and can be selected on Agar-plates deficient of uracil.
Notes
-Source
Based on invitrogen's pYES2-plasmid.| Sequence Annotation | Location | Component / Role(s) |
| T7-Promoter RFC25 MCS Strep-tag II CYC1-Terminator pUC-origin AmpR URA3 2 micron (??) origin | 4,23 36,134 78,101 148,396 578,1251 1396,2256 2274,3381 3385,4856 | feature/primer_binding primer_binding_site sequence_feature feature/misc feature/tag tag stop_codon feature/stop sequence_feature feature/dna feature/cds CDS CDS feature/cds feature/dna sequence_feature |