BBa_K801001

BBa_K801001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K801001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Georg Sch??tzinger

Date created: 2012-09-20 11:00:00

Date modified: 2015-05-08 01:13:24

pTUM101 yeast shuttle vector with pTEF1 promoter

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K801001_sequence (Version 1)

Description

This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).

Notes

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible

Source

Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7-Promoter	4,23	primer_binding_site, feature/primer_binding
TEF1-Promoter	58,505	feature/promoter, promoter
CYC1-Terminator	540,787	stop_codon, feature/stop
pUC-origin	970,1643	sequence_feature, feature/dna
AmpR	1788,2647	feature/cds, CDS
URA3	2666,3773	feature/cds, CDS
2 micron (??) origin	3777,5248	sequence_feature, feature/dna

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K801001/1

Member of these Collections

• //chassis/eukaryote/yeast
• //chassis/prokaryote/ecoli
• //plasmidbackbone/copynumber/high
• //plasmidbackbone/expression/constitutive
• iGEM Parts Registry
• igem_plasmid_vectors