Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Georg Sch??tzinger
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:24
pTUM102 yeast shuttle vector with pTEF2 promoter
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K801002_sequence (Version 1) |
Description
This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).Notes
All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.Source
Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).| Sequence Annotation | Location | Component / Role(s) |
| TEF2-Promoter CYC1-T pUC-origin AmpR URA3 2 micron (??) origin T7-Promoter | 58,705 739,984 1169,1842 1987,2846 2865,3972 3976,5447 4,23 | promoter feature/promoter stop_codon feature/stop sequence_feature feature/dna feature/cds CDS CDS feature/cds feature/dna sequence_feature feature/primer_binding primer_binding_site |