BBa_K801004

BBa_K801004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K801004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ingmar Polte

Date created: 2012-09-20 11:00:00

Date modified: 2015-05-08 01:13:24

pTUM104 yeast shuttle vector with GAL1 promoter

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K801004_sequence (Version 1)

Description

This part is based on the plasmid pTUM100 (BBa_K801000). The galactose-inducible GAL1 yeast promoter has been cloned in front of the MCS (RFC25 prefix and suffix).

Notes

All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.

Source

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CYC1 transcription terminator	44,292	feature/stop, stop_codon
pUC1 origin	474,1147	feature/misc, sequence_feature
Ampicillin	1292,2152	sequence_feature, feature/misc
URA3	2170,3277	sequence_feature, feature/misc
2 ?? origin	3281,4752	feature/misc, sequence_feature
f1 origin	4820,5275	feature/misc, sequence_feature
PGAL1	5293,5743	feature/promoter, promoter
T7 promoter/priming site	5767,5786	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K801004/1

Member of these Collections

• //chassis/eukaryote/yeast
• //chassis/prokaryote/ecoli
• //plasmidbackbone/copynumber/high
• //plasmidbackbone/expression/inducible
• iGEM Parts Registry
• igem_plasmid_vectors