BBa_K801042

BBa_K801042 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K801042

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dong-Jiunn Jeffery TRUONG

Date created: 2012-09-20 11:00:00

Date modified: 2015-05-08 01:13:24

GAL4 based yeast light-switchable promoter system

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K801042_sequence (Version 1)

Description

composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011

Notes

-

Source

composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K319003 (pTEF1, TEF1 promoter)	1,450	promoter, feature/promoter
BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)	456,1259	CDS, feature/cds
SV40 NLS (SV40 nuclear localization signal)	465,521	tag, feature/tag
GAL4AD (GAL4 transcriptonal activation domain)	525,863	feature/protein, CDS
Linker	864,959	feature/tag, tag
T7 promoter (for sequencing)	869,891	feature/promoter, promoter
HA epitope tage	912,932	feature/tag, tag
PIF3 (First 100 N-terminal amino acids)	960,1256	feature/protein, CDS
BBa_K801011, (tTEF1, TEF1 terminator)	1268,1774	sequence_feature, feature/misc
BBa_K319003 (pTEF1, TEF1 promoter)	1782,2231	promoter, feature/promoter
SV40NLS-PhyB-Linker-GAL4DBD	2237,5548	feature/cds, CDS
SV40 NLS (SV40 nuclear localization signal)	2246,2302	tag, feature/tag
BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)	2246,5539	engineered_region, feature/BioBrick
Phytochrome B (PhyB, 908 first N-terminal amino acids)	2309,5029	feature/protein, CDS
20 amino acids long linker (BBa_K365005)	5036,5095	feature/tag, tag
GAL4DBD (GAL4 DNA binding domain)	5102,5539	feature/protein, CDS
BBa_K801011, (tTEF1, TEF1 terminator)	5557,6063	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K801042/1

Member of these Collections

• //chassis/eukaryote/yeast
• //promoter
• //proteindomain
• //proteindomain/activation
• //proteindomain/binding
• //proteindomain/dnabinding
• //proteindomain/localization
• iGEM Parts Registry