Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dennis Hell
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:24
Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K801077_sequence (Version 1) |
Description
This part allows the expression of the three enzymes CaXMT1, CaMXMT1 and CaDXMT1 in yeast. These enzymes form the biosynthesis pathway for caffeine.The expression of the enzymes CaXMT1 (Coffea arabica xanthosine-N-methyl-transferase 1, BBa_K801070) and CaDXMT1 (Coffea arabica dimethyl-xanthine N-methyl transferase 1, = caffeine synthase, BBa_K801072) is controlled by the TEF2-promoter (BBa_K801010) and the ADH1-terminator (BBa_K801012)
The expression of CaMXMT1 (Coffea arabica methyl-xanthine N-methyl transferase 1, BBa_K801071) is controlled by the TEF1 promoter (BBa_K319003) and the ADH1-terminator (BBa_K801012)
Notes
-Source
Composite part of BBa_K801010 BBa_K801070 BBa_K801012 BBa_K319003 BBa_K801071 BBa_K801012 BBa_K801010 BBa_K801072 BBa_K801012| Sequence Annotation | Location | Component / Role(s) |
| Cassette1 + Cassette 2+ Cassette 3 Tef2 promoter CaXMT1 ADH1 terminator Tef1 promoter CaMXMT1 ADH1 terminator Tef2 promoter CaDXMT1 ADH1 terminator Consensus sequence Strep-Tag Stop codon Consensus sequence Strep-Tag Stop codon Consesus sequence Strep-Tag Stop codon Start codon Start codon Start codon | 1,6387 1,648 663,1778 1823,2172 2181,2631 2644,3777 3823,4171 4180,4827 4842,5993 6038,6387 657,662 1779,1808 1809,1814 2638,2643 3778,3807 3808,3813 4836,4841 5994,6023 6024,6029 663,665 2644,2646 4842,4844 | feature/BioBrick engineered_region promoter feature/promoter feature/cds CDS sequence_feature feature/misc promoter feature/promoter feature/cds CDS sequence_feature feature/misc feature/promoter promoter feature/cds CDS sequence_feature feature/misc feature/misc sequence_feature tag feature/tag feature/stop stop_codon sequence_feature feature/misc tag feature/tag feature/stop stop_codon feature/misc sequence_feature feature/tag tag feature/stop stop_codon start_codon feature/start start_codon feature/start start_codon feature/start |