BBa_K806002

BBa_K806002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K806002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chang Ye

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:25

hda - regulator of DnaA that prevents premature reinitiation of DNA replication

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K806002_sequence (Version 1)

Description

We find a better way to slow down the cell cycle of E.coli,by stop the initiation of DNA replication.
Hda is a great candidate for stop replication initiation.[1]
we cloned and overexpressed it to slow down certain kind of cells in a mix quorum system, in order to maintain a more stable system and achieve better energy partition.

[1] Elliott Crooke et al.EMBO report. Hda inactivation of DnaA is the predominant mechanism preventing hyperinitiation of Escherichia coli DNA replication.

Notes

1. Add restriction enzyme site on both end
2. clone the gene from genomic DNA extraction for better effect.

Source

we clone the gene in BL21 strain of E.coli, by using primer hda-F and hda-R
hda-F sequence: GCTCTAGAGCCTGAACACACCGGCACAGCTCTCT
hda-R sequence: GGACTAGTCCCTACAACTTCAGAATTTCTTTCACAAACGGA
hda genebank id: AM946981.2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
hda	1,747	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K806002/1

Member of these Collections

• iGEM Parts Registry