BBa_K806003

BBa_K806003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K806003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chang Ye

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:25

SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K806003_sequence (Version 1)

Description

We find a better way to slow down the cell cycle of E.coli,by stop the initiation of DNA replication.
iciA is ???the gene encoding the protein that binds the three 13-mers in the origin (oriC) of Escherichia coli to block initiation of replication in vitro???
we cloned and overexpressed it to slow down certain kind of cells in a mix quorum system, in order to maintain a more stable system and achieve better energy partition.

Notes

1. Add restriction enzyme site on both end
2. clone the gene from genomic DNA extraction for better effect.

Source

We clone this Gene from E.coli strain BL21 with primer seqA-F and seq-R
seqA-F sequence: GCTCTAGAGCATGAAAACGATTGAAGTTGATG
seqA-R sequence: GGACTAGTCCTTAGATAGTTCCGCAAACCTTC

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SeqA	1,546	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P0AFY8
• rdf-schema#seeAlso: P0AFY9
• rdf-schema#seeAlso: P0AFZ0
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K806003/1

Member of these Collections

• iGEM Parts Registry