BBa_K806004

BBa_K806004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K806004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chang Ye

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:25

cspD - stationary phase/starvation inducible regulatory protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K806004_sequence (Version 1)

Description

We find a better way to slow down the cell cycle of E.coli,by stop the initiation of DNA replication.
CsdD effect-ively inhibits both the initiation and the elongation steps of minichromosome replication in vitro.[1]
we cloned and overexpressed it to slow down certain kind of cells in a mix quorum system, in order to maintain a more stable system and achieve better energy partition.

[1]Yamanaka, K. Zheng, W. Crooke, E. Wang, Y.-H. Inouye, M. CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli. MOLECULAR MICROBIOLOGY. 2001, VOL 39; PART 6, pages 1572-1584.

Notes

1. Add restriction enzyme site on both end
2. clone the gene from genomic DNA extraction for better effect.

Source

We clone this gene in E.coli strain BL21 with primer cspD-F and cspD-R.
cspD-F sequence：GCTCTAGAGCATGGAAAAGGGTACTGTTAAGTGGTT
cspD-R sequence：GGACTAGTCCCTATGCGACTGCCGCTTCTACTT
cspD genebank id：AM946981.2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CspD	1,225	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K806004/1

Member of these Collections

• iGEM Parts Registry