LexA DBD

BBa_K807001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K807001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ian Roney

Date created: 2012-09-27 11:00:00

Date modified: 2015-05-08 01:13:25

Yeast Codon Optimized LexA DNA Binding Domain

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K807001_sequence (Version 1)

Description

Synthesized piece of DNA from IDT that was yeast codon optimized for optimal functioning. The first 252bp of the LexA repressor corresponding to the DNA binding domain were synthesized.

Notes

Had to take the LexA CDS and design primers to amplify the DNA binding domain only with no stop codon.

Source

Sequence was derived from the E.coli LexA repressor.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LexA DBD	1,252	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K807001/1

Member of these Collections

• iGEM Parts Registry