LEV

BBa_K807002 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K807002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ian Roney

Date created: 2012-09-27 11:00:00

Date modified: 2015-05-08 01:13:25

LEV: LexA DNA Binding Domain - Human Estrogen Receptor - VP16 Activating Domain

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K807002_sequence (Version 1)

Description

This part is a synthetic transcriptional activator with specificity for the LexA operator site. It is inactive in its native form but can be activated by the addition of Beta-Estradiol. The Beta-Estradiol acts to dimerize the activator.

Notes

LexA DBD was yeast optimized and synthesized by IDT. We had to ensure that LexA DBD and hER did not have stop codons in order for the fusion protein to be built.

Source

LexA DBD - E.coli
hER - Human
VP16 - Herpes virus

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LexA DBD	1,252	feature/binding, non_covalent_binding_site
hER	253,1135	feature/protein, CDS
VP16	1136,1339	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K807002/1

Member of these Collections

• iGEM Parts Registry