Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
Date created: 2012-09-01 11:00:00
Date modified: 2015-05-08 01:13:26
RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K808030_sequence (Version 1) |
Description
RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.Notes
We got it synthesized by GENEART but added the RBS via PCR.Source
This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0029 6 histag BBa_K808028 BBa_K808026 myctag BBa_K808027 stop stop start | 2,16 86,103 23,85 104,1573 1574,1603 1604,3037 3038,3040 3041,3043 23,25 | ribosome_entry_site feature/rbs tag feature/tag CDS feature/protein feature/protein CDS tag feature/tag CDS feature/protein stop_codon feature/stop stop_codon feature/stop start_codon feature/start |