Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Spencer R Katz
Date created: 2012-09-27 11:00:00
Date modified: 2015-05-08 01:13:27
Lambda phage beta CDS with T5 promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K810000_sequence (Version 1) |
Description
This part features the coding sequence for the recombinase of the bacteriophage lambda, called beta. It has been fused with a promoter designed by Bryksin and Matsumura (2010) that has been shown to promote transcription in both Gram-positive and Gram-negative organisms. It is flanked by XbaI and SpeI and features a KpnI restriction enzyme site between the promoter and coding sequence. It was designed to go in plasmid pBAV1K (Bryksin and Matsumura) which replicates in both Gram-positive and Gram-negative bacteria.Notes
The CDS for beta is downstream of the T5-lac promoter from plasmid pBAV1K-T5 described by Bryksin and Matsumura, 2010. The construct was designed to be cloned into pBAV1K to create a construct that could be used across species.Source
The promoter region was amplified from the plasmid pBAV1K-T5-gfp (Bryksin and Matsumura, 2010). The coding sequence was amplified from E. coli strain SIMD50 as described in Datta, et al., 2006. This coding sequence is originally from the bacteriophage lambda.| Sequence Annotation | Location | Component / Role(s) |
| bet CDS RBS T5 promoter -35 signal -10 signal LacO LacO KpnI restriction enzyme site | 83,868 65,76 1,57 1,7 24,31 7,25 39,57 77,82 | feature/cds CDS feature/rbs ribosome_entry_site promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature |