Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyrille Pauthenier
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:27
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K812012_sequence (Version 1) |
Description
OsTiRI is an ubuquitinase E3 that recognise and trigger the ubiquitination of every AID tagged protein. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
This part contains 3 myc domain for protein staining using antiMyc antibodies
Notes
This part has been cloned in pSB1C3 using in-fusion and the illegal restriction site SpeI and PstI had been removed by PCR.Source
This part has been extracted from the pNHK60 plasmid using PCR. THe pNHK60 plasmid was obtained from the riken registry.| Sequence Annotation | Location | Component / Role(s) |
| kozac OsTirI Myc Myc Myc PstI eliminated SpeI elimitated | 1,7 8,1732 1757,1792 1793,1828 1829,1864 1066,1066 1156,1156 | start_codon feature/start feature/protein CDS feature/protein CDS feature/protein CDS CDS feature/protein feature/s_mutation point_mutation point_mutation feature/s_mutation |