BBa_K812052

BBa_K812052 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K812052

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cyrille Pauthenier

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:27

pLac promoter R0010 with GoldenBrick extension

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K812052_sequence (Version 1)

Description

This part is the GoldenBricked version of R0010 pLac promoter. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.

To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB

Notes

The part R0010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.

Source

This part has been created from R0010 by PCR amplification

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812052/1

Member of these Collections

• iGEM Parts Registry