BBa_K812054

BBa_K812054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K812054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cyrille Pauthenier

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:27

RFP E1010 with GoldenBrick sequences

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K812054_sequence (Version 1)

Description

RFP E1010 with GoldenBrick extension

This part is the GoldenBricked version of E1010 RFP. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.

To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB

Notes

This part has been created from E1010 by PCR amplification

Source

The part E1010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GoldenBrick mRFP1 suffix	699,722	engineered_region, feature/BioBrick
mRFP1	18,692	feature/cds, CDS
GoldenBrick mRFP1 prefix	1,17	engineered_region, feature/BioBrick
OH3	14,17	feature/misc, sequence_feature
OH4	701,704	feature/misc, sequence_feature
EcoRI	1,6	feature/misc, sequence_feature
BsaI	7,12	feature/misc, sequence_feature
XbaI	13,17	sequence_feature, feature/misc
BbsI	699,704	feature/misc, sequence_feature
BsaI	706,711	sequence_feature, feature/misc
SpeI	712,717	sequence_feature, feature/misc
PstI	718,722	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812054/1

Member of these Collections

• iGEM Parts Registry