BBa_K814002

BBa_K814002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K814002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:28

dehydroquinate O-methyltrasferase (O-MT) generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K814002_sequence (Version 1)

Description

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. Dehydroquinate O-methyltransferase (O-MT) catalyzes the second step in this pathway, converting dehydroquinate to 4-deoxygadusol.

This part includes a modified constitutive lac promoter (lacPmod), and RBS and the open reading frame of O-MT. This part can be used to express O-MT in E. coli.

Notes

Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites.

Source

Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacP'	1,127	promoter, feature/promoter
RBS	128,133	ribosome_entry_site, feature/rbs
O-MT	140,980	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K814002/1

Member of these Collections

• iGEM Parts Registry