BBa_K814008

BBa_K814008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K814008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:28

ScyB (leucine dehydrogenase) generator

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K814008_sequence (Version 1)

Description

Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB (NpR1275), an acetolactate synthetase, carries out the first step in scytonemin biosynthesis, converting L-tryptophan to alpha-keto-tryptophan.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyB. This part can be used to express ScyB protein in E. coli.

Notes

N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS.

Source

Nostoc punctiforme PCC 73102, accession NC_010628.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacP'	1,127	feature/promoter, promoter
RBS	128,133	feature/rbs, ribosome_entry_site
ScyB	140,1201	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K814008/1

Member of these Collections

• iGEM Parts Registry