BBa_K814009

BBa_K814009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K814009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Bloch

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:28

ScyC generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K814009_sequence (Version 1)

Description

Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyC (NpR1276) is a protein of unknown function which converts 3-indole pyruvic acid to a ketoacid intermediate which spontaneously dimerizes to form scytonemin.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli.

Notes

N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS.

Source

Nostoc punctiforme PCC 73102, accession NC_010628.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacP'	1,127	promoter, feature/promoter
RBS	128,133	feature/rbs, ribosome_entry_site
ScyC	140,2014	feature/protein, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K814009/1

Member of these Collections

• iGEM Parts Registry