| Types | DnaRegion
|
| Roles | Device
engineered_region
|
| Sequences | BBa_K817015_sequence (Version 1)
|
Description
It is type I toxin-antitoxin which belongs to hok/sok homologues, it expresses stable toxin encoded RNA and short lived antitoxin RNA that can neutralize toxin RNA by RNA interaction. It acts as post segregation killing system, which kills bacteria when it loss the DNA(genomic islands, plasmids, mobile gene elements) that contains it. Therefore we use it to reduce plasmid loss rate and make applications without antibiotics selection pressure more feasible.
Notes
This part only functions when there is not the same srnBC present in the same cell(those F' E.coli like JM109 contain srnBC), or it will be neutralize and functionless .
Source
F plasmid
