Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Chi Hsieh
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:28
pSB2K3 with parS site
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K817017_sequence (Version 1) |
Description
pSB2K3 is mini F plasmid that lacks partition system and replicates as low copy in cell. pSB2K3 with parS site can be partitioned by parAB(BBa_K817007) cassette which can enhances its segregation stability.Notes
parS site is put into biobricks cloning site not on backbone.Source
pseudomonas putida KT2440 chromosome| Sequence Annotation | Location | Component / Role(s) |
| suffix E. coli his operon terminator terminator stem loop deletion of two bases VR F1 Replication Origin repE (rev) oriS Replication Origin (rev) lacOP repL nnpp promoters kanR VF2 end of terminator terminator stem loop prefix parB binding site (parS) | 1,21 22,93 30,64 34,35 157,176 242,290 617,1372 1463,1679 2037,2211 2289,3134 3242,3393 3433,4248 4286,4305 4362,4365 4375,4394 4404,4425 4426,4441 | feature/BioBrick engineered_region feature/misc sequence_feature stem_loop feature/stem_loop sequence_feature feature/misc primer_binding_site feature/primer_binding feature/promoter promoter feature/cds CDS promoter feature/promoter operator feature/operator CDS feature/cds promoter feature/promoter CDS feature/cds primer_binding_site feature/primer_binding feature/misc sequence_feature stem_loop feature/stem_loop engineered_region feature/BioBrick non_covalent_binding_site feature/binding |